Proteintech, 11097-1-AP, c-SRC Polyclonal antibody

Size: 20ul / 150ul The c-SRC (11097-1-AP) by Proteintech is a Polyclonal antibody targeting c-SRC in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, hamster samples 11097-1-AP targets c-SRC in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples. Tested Applications Positive WB detected in: HUVEC cells, mouse brain tissue, COLO 320 cells, Jurkat cells, A431 cells, CHO cells, SH-SY5Y cells Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human breast cancer tissue, human brain tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171). Specification Tested Reactivity: human, mouse, rat, hamster Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1575 Product name: Recombinant human c-SRC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 187-536 aa of BC011566 Sequence: YCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL Predict reactive species Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 55 kDa, 60 kDa GenBank Accession Number: BC011566 Gene Symbol: SRC Gene ID (NCBI): 6714 ENSEMBL Gene ID: ENSG00000197122 RRID: AB_2286705 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P12931 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.