Proteintech, 11116-1-AP, CTDSP2 Polyclonal antibody

Size: 20ul / 150ul The CTDSP2 (11116-1-AP) by Proteintech is a Polyclonal antibody targeting CTDSP2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11116-1-AP targets CTDSP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human kidney tissue, HepG2 cells, K-562 cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information CTDSPs are C-class phosphatases that act on Ser 5 of the Pol II C-terminal domain (CTD) to decrease transcription [PMID:15681389]. CTDSP enzymatic activity is required for REST function, and knockdown of CTDSP induces the neuronal differentiation of mouse embryonic carcinoma P19 cells. CTDSP2 has been shown to enhance TGF-β signaling by the dephosphorylation of SMAD proteins and attenuates androgen receptor-mediated transcription [PMID:16724108]. It negatively regulates RNA polymerase II transcription by controlling the transition from initiation/capping to processive transcript elongation. It was recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells[PMID: 12721286]. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1601 Product name: Recombinant human CTDSP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC065920 Sequence: MEHGSIITQARREDALVLTKQGLVSKSSPKKPRGRNIFKALFCCFRAQHVGQSSSSTELAAYKEEANTIAKSDLLQCLQYQFYQIPGTCLLPEVTEEDQGRICVVIDLDETLVHSSFKPINNADFIVPIEIEGTTHQVYVLKRPYVDEFLRRMGELFECVLFTASLAKYADPVTDLLDRCGVFRARLFRESCVFHQGCYVKDLSRLGRDLRKTLILDNSPASYIFHPENAVPVQSWFDDMADTELLNLIPIFEELSGAEDVYTSLGQLRAP Predict reactive species Full Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC065920 Gene Symbol: CTDSP2 Gene ID (NCBI): 10106 RRID: AB_2087086 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14595 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.