Proteintech, 11171-1-AP, PRPF8 Polyclonal antibody

Size: 20ul / 150ul The PRPF8 (11171-1-AP) by Proteintech is a Polyclonal antibody targeting PRPF8 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 11171-1-AP targets PRPF8 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, HeLa cells, human placenta tissue Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1652 Product name: Recombinant human PRPF8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2037-2335 aa of BC064370 Sequence: SQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA Predict reactive species Full Name: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) Calculated Molecular Weight: 274 kDa Observed Molecular Weight: 220 kDa GenBank Accession Number: BC064370 Gene Symbol: PRPF8 Gene ID (NCBI): 10594 RRID: AB_2171179 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6P2Q9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.