Proteintech, 11256-1-AP, Annexin A2 Polyclonal antibody

Size: 20ul / 150ul The Annexin A2 (11256-1-AP) by Proteintech is a Polyclonal antibody targeting Annexin A2 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 11256-1-AP targets Annexin A2 in WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, mouse placenta tissue, THP-1 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse placenta tissue, human bowen disease tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: K-562 cells, A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Predict reactive species Full Name: annexin A2 Calculated Molecular Weight: 339 aa, 39 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC016774 Gene Symbol: Annexin A2 Gene ID (NCBI): 302 ENSEMBL Gene ID: ENSG00000182718 RRID: AB_2057311 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07355 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.