Proteintech, 11344-1-AP, HDGF Polyclonal antibody

Size: 20ul / 150ul The HDGF (11344-1-AP) by Proteintech is a Polyclonal antibody targeting HDGF in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11344-1-AP targets HDGF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293T cells, Hela cells Positive IP detected in: HepG2 cells Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue, human liver cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information HDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like) Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC018991 Gene Symbol: HDGF Gene ID (NCBI): 3068 RRID: AB_2232950 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51858 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.