Proteintech, 11353-1-AP, MYDGF/C19orf10 Polyclonal antibody

Size: 20ul / 150ul The MYDGF/C19orf10 (11353-1-AP) by Proteintech is a Polyclonal antibody targeting MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: DU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1900 Product name: Recombinant human C19orf10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC010129 Sequence: MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL Predict reactive species Full Name: chromosome 19 open reading frame 10 Calculated Molecular Weight: 173 aa, 19 kDa Observed Molecular Weight: 15-19 kDa GenBank Accession Number: BC010129 Gene Symbol: MYDGF/C19orf10 Gene ID (NCBI): 56005 RRID: AB_2207647 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969H8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.