Proteintech, 11398-1-AP, POF1B Polyclonal antibody

Size: 20ul / 150ul The POF1B (11398-1-AP) by Proteintech is a Polyclonal antibody targeting POF1B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11398-1-AP targets POF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, mouse colon tissue Positive IHC detected in: human ovary cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information POF1B plays a key role in the organization of epithelial monolayers by regulating the actin cytoskeleton. POF1B is a candidate gene for premature ovarian failure (POF); it is mainly expressed in polarised epithelial tissues. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1945 Product name: Recombinant human POF1B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 247-595 aa of BC017500 Sequence: DGPEKLDPRYFGELLADLSRKNTDLYHCLLEHLQRIGGSKQDFESTDESEDIESLIPKGLSEFTKQQIRYILQMRGMSDKSLRLVLSTFSNIREELGHLQNDLTSLENDKMRLEKDLSFKDTQLKEYEELLASVRANNHQQQQGLQDSSSKCQALEENNLSLRHTLSDMEYRLKELEYCKRNLEQENQNLRMQVSETCTGPMLQAKMDEIGNHYTEMVKNLRMEKDREICRLRSQLNQYHKDVSKREGSCSDFQFKLHELTSLLEEKDSLIKRQSEELSKLRQEIYSSHNQPSTGGRTTITTKKYRTQYPILGLLYDDYEYIPPGSETQTIVIEKTEDKYTCVSSLGHF Predict reactive species Full Name: premature ovarian failure, 1B Calculated Molecular Weight: 595 aa, 69 kDa Observed Molecular Weight: 69 kDa GenBank Accession Number: BC017500 Gene Symbol: POF1B Gene ID (NCBI): 79983 RRID: AB_2252552 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WVV4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.