Proteintech, 11427-1-AP, ANKRD1 Polyclonal antibody

Size: 20ul / 150ul The ANKRD1 (11427-1-AP) by Proteintech is a Polyclonal antibody targeting ANKRD1 in WB, IF-P, ELISA applications with reactivity to human, mouse, rat samples 11427-1-AP targets ANKRD1 in WB, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression. This is a rabbit polyclonal antibody produced by the full-length ANKRD1 of human origin Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1970 Product name: Recombinant human ANKRD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-319 aa of BC018667 Sequence: MMVLKVEELVTGKKNGNGEAGEFLPEDFRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAELKKKKLEQRSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKAALENKLPVVEKFLSDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWASRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGADLNIKNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF Predict reactive species Full Name: ankyrin repeat domain 1 (cardiac muscle) Calculated Molecular Weight: 319 aa, 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC018667 Gene Symbol: ANKRD1 Gene ID (NCBI): 27063 RRID: AB_2227402 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.