Proteintech, 11472-1-AP, Calpain 2 Polyclonal antibody

Size: 20ul / 150ul The Calpain 2 (11472-1-AP) by Proteintech is a Polyclonal antibody targeting Calpain 2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11472-1-AP targets Calpain 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, MDA-MB-231 cells, human placenta tissue, HeLa cells, Y79 cells, C2C12 cells, mouse brain tissue, rat brain tissue, K-562 cells, PC-3 cells Positive IP detected in: MDA-MB-231 cells Positive IHC detected in: human kidney tissue, human colon cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2029 Product name: Recombinant human CAPN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC021303 Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD Predict reactive species Full Name: calpain 2, (m/II) large subunit Calculated Molecular Weight: 700 aa, 80 kDa Observed Molecular Weight: 72-80 kDa GenBank Accession Number: BC021303 Gene Symbol: CAPN2 Gene ID (NCBI): 824 RRID: AB_10755306 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P17655 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.