Proteintech, 11525-1-AP, MCC Polyclonal antibody

Size: 20ul / 150ul The MCC (11525-1-AP) by Proteintech is a Polyclonal antibody targeting MCC in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 11525-1-AP targets MCC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: fetal human brain tissue, HeLa cells, human brain tissue, L02 cells, mouse liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin, Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2089 Product name: Recombinant human MCC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 480-829 aa of BC018919 Sequence: AGVGSSPGDQSGDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMAELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADAASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL Predict reactive species Full Name: mutated in colorectal cancers Calculated Molecular Weight: 829 aa, 93 kDa Observed Molecular Weight: 98 kDa, 113-118 kDa GenBank Accession Number: BC018919 Gene Symbol: MCC Gene ID (NCBI): 4163 RRID: AB_2143796 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P23508 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.