Proteintech, 11534-1-AP, Osteoprotegerin/TNFRSF11B Polyclonal antibody

Size: 20ul / 150ul The Osteoprotegerin/TNFRSF11B (11534-1-AP) by Proteintech is a Polyclonal antibody targeting Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 11534-1-AP targets Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human milk Positive IHC detected in: rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promoting bone formationis. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2139 Product name: Recombinant human TNFRSF11B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC030155 Sequence: MNNLLCCALVFLDISIKWTTQETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQKCG Predict reactive species Full Name: tumor necrosis factor receptor superfamily, member 11b Calculated Molecular Weight: 401 aa, 46 kDa Observed Molecular Weight: 56 kDa GenBank Accession Number: BC030155 Gene Symbol: TNFRSF11B Gene ID (NCBI): 4982 RRID: AB_3085376 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00300 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.