Proteintech, 11537-1-AP, LRRC8D Polyclonal antibody

Size: 20ul / 150ul The LRRC8D (11537-1-AP) by Proteintech is a Polyclonal antibody targeting LRRC8D in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11537-1-AP targets LRRC8D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HEK-293 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2138 Product name: Recombinant human LRRC8D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 515-858 aa of BC024159 Sequence: LQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI Predict reactive species Full Name: leucine rich repeat containing 8 family, member D Calculated Molecular Weight: 858 aa, 98 kDa Observed Molecular Weight: 98-120 kDa GenBank Accession Number: BC024159 Gene Symbol: LRRC8D Gene ID (NCBI): 55144 RRID: AB_2137767 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7L1W4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.