Proteintech, 11542-1-AP, ORC5L Polyclonal antibody

Size: 20ul / 150ul The ORC5L (11542-1-AP) by Proteintech is a Polyclonal antibody targeting ORC5L in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11542-1-AP targets ORC5L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, human kidney tissue, mouse ovary tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Origin recognition complex (ORC) plays an essential role in the initiation of chromosome DNA replication and transcriptional silencing. ORC binds in an ATP-dependent manner to several well-defined autonomously replicating sequences that serve as the chromosomal replication origins. ORC5L is one of the 6 subunits of ORC. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2133 Product name: Recombinant human ORC5L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 127-435 aa of BC023652 Sequence: AEYLRDMEANLLPGFLRLQELADRNVTVLFLSEIVWEKFRPNTGCFEPFVLYFPDYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTVCRDLKELRHLAVLNFPKYCEPVVKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSSQWEKLQKDDTDPGQLKGLSAHTHVELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLDRLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTLVGHDDQLDGPKYKCTVSLDFIRAIARTVNFDIIKYLYDFL Predict reactive species Full Name: origin recognition complex, subunit 5-like (yeast) Calculated Molecular Weight: 435 aa, 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC023652 Gene Symbol: ORC5L Gene ID (NCBI): 5001 RRID: AB_2267695 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43913 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.