Proteintech, 11580-1-AP, TNS4 Polyclonal antibody

Size: 20ul / 150ul The TNS4 (11580-1-AP) by Proteintech is a Polyclonal antibody targeting TNS4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11580-1-AP targets TNS4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2169 Product name: Recombinant human TNS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 130-480 aa of BC018706 Sequence: ITNSMVDIPIVLINGCPEPGSSPPQRTPGHQNSVQPGAASPSNPCPATRSNSQTLSDAPFTTCPEGPARDMQPTMKFVMDTSKYWFKPNITREQAIELLRKEEPGAFVIRDSSSYRGSFGLALKVQEVPASAQSRPGEDSNDLIRHFLIESSAKGVHLKGADEEPYFGSLSAFVCQHSIMALALPCKLTIPQRELGGADGASDSTDSPASCQKKSAGCHTLYLSSVSVETLTGALAVQKAISTTFERDILPTPTVVHFKVTEQGITLTDVQRKVFFRRHYPLTTLRFCGMDPEQRKWQKYCKPSWIFGFVAKSQTEPQENVCHLFAEYDMVQPASQVIGLVTALLQDAERM Predict reactive species Full Name: tensin 4 Calculated Molecular Weight: 715 aa, 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: BC018706 Gene Symbol: TNS4 Gene ID (NCBI): 84951 RRID: AB_10640582 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IZW8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.