Proteintech, 11582-1-AP, PLSCR1 Polyclonal antibody

Size: 20ul / 150ul The PLSCR1 (11582-1-AP) by Proteintech is a Polyclonal antibody targeting PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications with reactivity to human, mouse, rat samples 11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse lung tissue, A549 cells, HepG2 cells, human placenta tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: mouse spleen tissue, human kidney tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: rat brain tissue, mouse brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2172 Product name: Recombinant human PLSCR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC021100 Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW Predict reactive species Full Name: phospholipid scramblase 1 Calculated Molecular Weight: 318 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC021100 Gene Symbol: PLSCR1 Gene ID (NCBI): 5359 RRID: AB_2165659 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15162 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.