Proteintech, 11586-1-AP, PUM2 Polyclonal antibody

Size: 20ul / 150ul The PUM2 (11586-1-AP) by Proteintech is a Polyclonal antibody targeting PUM2 in WB, ELISA applications with reactivity to human, mouse, rat samples 11586-1-AP targets PUM2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, rat testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information PUM2 (Human Pumilio homology protein 2), a member of the PUF protein family, binds to 3′ untranslated regions (3′ UTRs) of mRNA and to repress gene expression by controlling cytoplasmic polyadenylation and affecting mRNA translation [PMID:12459267]. When translationally dormant, PUM2, CPEB (CPE-binding protein) and Maskin form a complex on maternal cyclin B1 mRNA, precluding the formation of the active translation initiation complex [PMID:15687499]. PUM2 has been found to play a role in the mammalian nervous system, in particular in regulating morphology, arborization and excitability of neuronal dendrites [PMID:22016787]. The calculated molecular weight of PUM2 is 114 kDa, but the modified PUM2 protein is about 140 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2179 Product name: Recombinant human PUM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 140-489 aa of BC024218 Sequence: NRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGPPNGML Predict reactive species Full Name: pumilio homolog 2 (Drosophila) Calculated Molecular Weight: 114 kDa Observed Molecular Weight: 95 kDa, 140 kDa GenBank Accession Number: BC024218 Gene Symbol: PUM2 Gene ID (NCBI): 23369 RRID: AB_2284639 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TB72 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.