Proteintech, 11633-1-AP, ZMYND8 Polyclonal antibody

Size: 20ul / 150ul The ZMYND8 (11633-1-AP) by Proteintech is a Polyclonal antibody targeting ZMYND8 in WB, IHC, IP, ELISA applications with reactivity to human samples 11633-1-AP targets ZMYND8 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins, including REST corepressors (RCOR). ZMYND8 binds to ROCR2 and can function as a transcriptional repressor. It is co-expressed with RCOR2 in the nervous system, and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974] Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2207 Product name: Recombinant human ZMYND8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 745-1094 aa of BC030721 Sequence: WNSSTVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD Predict reactive species Full Name: zinc finger, MYND-type containing 8 Calculated Molecular Weight: 1186 aa, 132 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC030721 Gene Symbol: ZMYND8 Gene ID (NCBI): 23613 RRID: AB_2241834 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULU4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.