Proteintech, 11640-1-AP, CDC6 Polyclonal antibody

Size: 20ul / 150ul The CDC6 (11640-1-AP) by Proteintech is a Polyclonal antibody targeting CDC6 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11640-1-AP targets CDC6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, SMMC-7721 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, human colon cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: sodium arsenite treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2228 Product name: Recombinant human CDC6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 217-560 aa of BC025232 Sequence: QDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEIDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP Predict reactive species Full Name: cell division cycle 6 homolog (S. cerevisiae) Calculated Molecular Weight: 560 aa, 63 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC025232 Gene Symbol: CDC6 Gene ID (NCBI): 990 RRID: AB_2078519 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99741 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.