Proteintech, 11641-1-AP, GNAI3 Polyclonal antibody

Size: 20ul / 150ul The GNAI3 (11641-1-AP) by Proteintech is a Polyclonal antibody targeting GNAI3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 11641-1-AP targets GNAI3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue Positive IHC detected in: human kidney tissue, human brain tissue, human skin cancer tissue, mouse cerebellum tissue, rat cerebellum tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2229 Product name: Recombinant human GNAI3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC025285 Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY Predict reactive species Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 Calculated Molecular Weight: 40 kDa Observed Molecular Weight: ~40 kDa GenBank Accession Number: BC025285 Gene Symbol: GNAI3 Gene ID (NCBI): 2773 RRID: AB_2111489 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08754 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.