Proteintech, 11870-1-AP, VPS37A Polyclonal antibody

Size: 20ul / 150ul The VPS37A (11870-1-AP) by Proteintech is a Polyclonal antibody targeting VPS37A in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 11870-1-AP targets VPS37A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2453 Product name: Recombinant human VPS37A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-174 aa of BC022363 Sequence: MPDVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSIEELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTFEKKMQRQHELSESCSASALQARLKVAAHEAEEESDNIAEDFLEGKMEIDDFLSSFMEKRTICHCRRAKEEKLQQAIAMHSQFHAPL Predict reactive species Full Name: vacuolar protein sorting 37 homolog A (S. cerevisiae) Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC022363 Gene Symbol: VPS37A Gene ID (NCBI): 137492 RRID: AB_2215230 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NEZ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.