Proteintech, 11906-1-AP, SCEL Polyclonal antibody

Size: 20ul / 150ul The SCEL (11906-1-AP) by Proteintech is a Polyclonal antibody targeting SCEL in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 11906-1-AP targets SCEL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human saliva tissue, A431 cells, human skin tissue, mouse skin tissue Positive IHC detected in: human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2578 Product name: Recombinant human SCEL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC020726 Sequence: MSNVTLRKMSPTGNEMKSTTQGTTRKQQDFHEVNKRRTFLQDNSWIKKRPEEEKDENYGRVVLNRHNSHDALDRKVNERDVPKATISRYSSDDTLDRSMSMFRSLEVTKLQPGGSLNANTSNTIASTSATTPVKKKRQSWFPPPPPGYNASSSTGTRRREPGVHPPIPPKPSSPVSSPNQLRQDNRQIHPPKPGVYTETNRSAERNISEELDNLIKMNKSLNRNQGLDSLFRANPKVEEREKKAKSLESLIYMSTRTDKDGKGIQSLGSPIKVNQRTDKNEKG Predict reactive species Full Name: sciellin Calculated Molecular Weight: 668 aa, 75 kDa Observed Molecular Weight: 70-78 kDa GenBank Accession Number: BC020726 Gene Symbol: SCEL Gene ID (NCBI): 8796 RRID: AB_2183262 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95171 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.