Proteintech, 11927-1-AP, ERGIC2 Polyclonal antibody

Size: 20ul / 150ul The ERGIC2 (11927-1-AP) by Proteintech is a Polyclonal antibody targeting ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11927-1-AP targets ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2603 Product name: Recombinant human ERGIC2 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-377 aa of BC000887 Sequence: MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH Predict reactive species Full Name: ERGIC and golgi 2 Calculated Molecular Weight: 377 aa, 43 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC000887 Gene Symbol: ERGIC2 Gene ID (NCBI): 51290 RRID: AB_2098440 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96RQ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.