Proteintech, 11946-1-AP, ATF1 Polyclonal antibody

Size: 20ul / 150ul The ATF1 (11946-1-AP) by Proteintech is a Polyclonal antibody targeting ATF1 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 11946-1-AP targets ATF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, human brain tissue Positive IP detected in: HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2548 Product name: Recombinant human ATF1 protein Source: e coli. -derived, T-GST Tag: GST Domain: 1-271 aa of BC029619 Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV Predict reactive species Full Name: activating transcription factor 1 Calculated Molecular Weight: 271 aa, 29 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC029619 Gene Symbol: ATF1 Gene ID (NCBI): 466 RRID: AB_2060777 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P18846 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.