Proteintech, 11962-1-AP, MCPH/BRIT1 Polyclonal antibody

Size: 20ul / 150ul The MCPH/BRIT1 (11962-1-AP) by Proteintech is a Polyclonal antibody targeting MCPH/BRIT1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue Positive IHC detected in: human prostate cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 ) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2567 Product name: Recombinant human BRIT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 253-610 aa of BC030702 Sequence: GSINDIKSDVCISSLVLKANNIHSSPSFTHLDKSSPQKFLSNLSKEEINLQRNIAGKVVTPHQKQAAGMSQETFEEKYRLSPTLSSTKGHLLIHSRPRSSSVKRKRVSHGSHSPPKEKCKRKRSTRRSIMPRLQLCRSEGRLQHVAGPALEALSCGESSYDDYFSPDNLKERYSENLPPESQLPSSPAQLSCRSLSKKERTSIFEMSDFSCVGKKTRTVDITNFTAKTISSPRKTGNGEGRATSSCVTSAPEEALRCCRQAGKEDACPEGNGFSYTIEDPALPKGHDDDLTPLEGSLEEMKEAVGLKSTQNKGTTSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKENLPGGYSGSM Predict reactive species Full Name: microcephalin 1 Calculated Molecular Weight: 93 kDa Observed Molecular Weight: 66 kDa, 100 kDa GenBank Accession Number: BC030702 Gene Symbol: MCPH1 Gene ID (NCBI): 79648 RRID: AB_2142989 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NEM0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.