Proteintech, 12067-1-AP, ATPIF1 Polyclonal antibody

Size: 20ul / 150ul The ATPIF1 (12067-1-AP) by Proteintech is a Polyclonal antibody targeting ATPIF1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 12067-1-AP targets ATPIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2704 Product name: Recombinant human ATPIF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC009677 Sequence: MAVTALAARTWLGVWGVRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD Predict reactive species Full Name: ATPase inhibitory factor 1 Calculated Molecular Weight: 106 aa, 12 kDa Observed Molecular Weight: 12 kDa GenBank Accession Number: BC009677 Gene Symbol: ATPIF1 Gene ID (NCBI): 93974 RRID: AB_2063159 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UII2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.