Proteintech, 12076-1-AP, USP48 Polyclonal antibody

Size: 20ul / 150ul The USP48 (12076-1-AP) by Proteintech is a Polyclonal antibody targeting USP48 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12076-1-AP targets USP48 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2749 Product name: Recombinant human USP48 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 782-1035 aa of BC013567 Sequence: MFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH Predict reactive species Full Name: ubiquitin specific peptidase 48 Calculated Molecular Weight: 1035 aa, 119 kDa Observed Molecular Weight: 119 kDa GenBank Accession Number: BC013567 Gene Symbol: USP48 Gene ID (NCBI): 84196 RRID: AB_2213840 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86UV5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.