Proteintech, 12078-1-AP, GNA15 Polyclonal antibody

Size: 20ul / 150ul The GNA15 (12078-1-AP) by Proteintech is a Polyclonal antibody targeting GNA15 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12078-1-AP targets GNA15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MOLT-4 cells, mouse spleen tissue Positive IHC detected in: human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GNA15, also known as GNA16, belongs to the G-alpha family and G(q) subfamily. The protein encoded by the GNA15 gene is the alpha subunit of the G protein, which participates in the regulation of cell proliferation and apoptosis. GNA15 is highly expressed in some cell types, such as hematopoietic stem cells and epithelial cells during specific stages of differentiation. GNA15 is known to play as an oncogene in several tumors, such as gastroenteropancreatic neuroendocrine neoplasia (GEP-NEN), liver cancer, pancreatic ductal adenocarcinoma and ovarian cancer (PMID: 34552875). The molecular weight of GNA15 is 44 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2738 Product name: Recombinant human GNA15 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-374 aa of BC013585 Sequence: MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRACYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL Predict reactive species Full Name: guanine nucleotide binding protein (G protein), alpha 15 (Gq class) Calculated Molecular Weight: 374 aa, 44 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC013585 Gene Symbol: GNA15 Gene ID (NCBI): 2769 RRID: AB_3085389 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30679 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.