Proteintech, 12171-1-AP, E2F1 Polyclonal antibody

Size: 20ul / 150ul The E2F1 (12171-1-AP) by Proteintech is a Polyclonal antibody targeting E2F1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12171-1-AP targets E2F1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, mouse brain tissue, NIH/3T3 cells, HepG2 cells, Jurkat cells, LNCaP cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:200 Background Information Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, artemia sinica Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2813 Product name: Recombinant human E2F1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 88-438 aa of BC050369 Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF Predict reactive species Full Name: E2F transcription factor 1 Calculated Molecular Weight: 437 aa, 47 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC050369 Gene Symbol: E2F1 Gene ID (NCBI): 1869 RRID: AB_2096958 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01094 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.