Proteintech, 12199-1-AP, USP25 Polyclonal antibody

Size: 20ul / 150ul The USP25 (12199-1-AP) by Proteintech is a Polyclonal antibody targeting USP25 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 12199-1-AP targets USP25 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: J774A.1 cells, BxPC-3 cells, mouse cerebellum tissue, K-562 cells, RAW 264.7 cells Positive IP detected in: RAW 264.7 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2837 Product name: Recombinant human USP25 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 171-451 aa of BC015930 Sequence: LKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR Predict reactive species Full Name: ubiquitin specific peptidase 25 Calculated Molecular Weight: 125 kDa Observed Molecular Weight: 125 kDa GenBank Accession Number: BC015930 Gene Symbol: USP25 Gene ID (NCBI): 29761 RRID: AB_2212771 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UHP3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.