Proteintech, 12208-1-AP, CREB1 Polyclonal antibody

Size: 20ul / 150ul The CREB1 (12208-1-AP) by Proteintech is a Polyclonal antibody targeting CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications with reactivity to human, mouse, rat, monkey samples 12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples. Tested Applications Positive WB detected in: HEK-293 cells, COS-7 cells, Calyculin A treated HEK-293 cells, HL-1 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, multi-cells/tissue, rat brain tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, WB result of FTO antibody (12208-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and CREB1 knockout HL-1 cells., PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HEK-293 cells Positive FC (Intra) detected in: HEK-293 cells Positive ChIP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Chromatin immunoprecipitation (ChIP): CHIP : 1:10-1:100 Background Information CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ). Specification Tested Reactivity: human, mouse, rat, monkey Cited Reactivity: human, mouse, rat, chicken, goat, sea cucumbers Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2852 Product name: Recombinant human CREB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC010636 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Predict reactive species Full Name: cAMP responsive element binding protein 1 Calculated Molecular Weight: 341 aa, 35 kDa Observed Molecular Weight: 43-46 kDa GenBank Accession Number: BC010636 Gene Symbol: CREB1 Gene ID (NCBI): 1385 RRID: AB_2245417 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16220 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.