Proteintech, 12214-1-AP, MINA Polyclonal antibody

Size: 20ul / 150ul The MINA (12214-1-AP) by Proteintech is a Polyclonal antibody targeting MINA in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12214-1-AP targets MINA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, mouse liver tissue, A375 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2838 Product name: Recombinant human MINA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 160-465 aa of BC014928 Sequence: SLVGSNVYITPAGSQGLPPHYDDVEVFILQLEGEKHWRLYHPTVPLAREYSVEAEERIGRPVHEFMLKPGDLLYFPRGTIHQADTPAGLAHSTHVTISTYQNNSWGDFLLDTISGLVFDTAKEDVELRTGIPRQLLLQVESTTVATRRLSGFLRTLADRLEGTKELLSSDMKKDFIMHRLPPYSAGDGAELSTPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDETQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFPLSHLDALKQIWNSPAISVKDLKLTTDEEKESLVLSLWTECLIQVV Predict reactive species Full Name: MYC induced nuclear antigen Calculated Molecular Weight: 465 aa, 53 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC014928 Gene Symbol: MINA Gene ID (NCBI): 84864 RRID: AB_2144191 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IUF8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.