Proteintech, 12243-1-AP, LCAT Polyclonal antibody

Size: 20ul / 150ul The LCAT (12243-1-AP) by Proteintech is a Polyclonal antibody targeting LCAT in WB, ELISA applications with reactivity to human, mouse, rat samples 12243-1-AP targets LCAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2887 Product name: Recombinant human LCAT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 94-440 aa of BC014781 Sequence: LGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE Predict reactive species Full Name: lecithin-cholesterol acyltransferase Calculated Molecular Weight: 440 aa, 50 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC014781 Gene Symbol: LCAT Gene ID (NCBI): 3931 RRID: AB_10638476 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04180 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.