Proteintech, 12297-1-AP, Neurotrophin 4 Polyclonal antibody

Size: 20ul / 150ul The Neurotrophin 4 (12297-1-AP) by Proteintech is a Polyclonal antibody targeting Neurotrophin 4 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12297-1-AP targets Neurotrophin 4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue Positive IF/ICC detected in: PC-3 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2938 Product name: Recombinant human NTF4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC012421 Sequence: MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA Predict reactive species Full Name: neurotrophin 4 Calculated Molecular Weight: 210 aa, 22 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC012421 Gene Symbol: Neurotrophin 4 Gene ID (NCBI): 4909 RRID: AB_10598309 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P34130 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.