Proteintech, 12322-1-AP, Syntaxin 7 Polyclonal antibody

Size: 20ul / 150ul The Syntaxin 7 (12322-1-AP) by Proteintech is a Polyclonal antibody targeting Syntaxin 7 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, canine samples 12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive WB detected in: mouse spleen tissue, MDCK cells Positive IP detected in: mouse heart tissue Positive IHC detected in: human medulloblastoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, canine Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2975 Product name: Recombinant human STX7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC011975 Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI Predict reactive species Full Name: syntaxin 7 Calculated Molecular Weight: 261 aa, 30 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC011975 Gene Symbol: Syntaxin 7 Gene ID (NCBI): 8417 RRID: AB_2239979 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15400 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.