Proteintech, 12332-1-AP, IDH1 Polyclonal antibody

Size: 20ul / 150ul The IDH1 (12332-1-AP) by Proteintech is a Polyclonal antibody targeting IDH1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HAP1 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2986 Product name: Recombinant human IDH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 119-414 aa of BC012846 Sequence: RLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL Predict reactive species Full Name: isocitrate dehydrogenase 1 (NADP+), soluble Calculated Molecular Weight: 414 aa, 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC012846 Gene Symbol: IDH1 Gene ID (NCBI): 3417 RRID: AB_2123159 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75874 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.