Proteintech, 12374-1-AP, SNX12 Polyclonal antibody

Size: 20ul / 150ul The SNX12 (12374-1-AP) by Proteintech is a Polyclonal antibody targeting SNX12 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12374-1-AP targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3014 Product name: Recombinant human SNX12 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-162 aa of BC020559 Sequence: MSDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPGKSLA Predict reactive species Full Name: sorting nexin 12 Calculated Molecular Weight: 172 aa, 20 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC020559 Gene Symbol: SNX12 Gene ID (NCBI): 29934 RRID: AB_3085392 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UMY4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.