Proteintech, 12391-1-AP, CA8 Polyclonal antibody

Size: 20ul / 150ul The CA8 (12391-1-AP) by Proteintech is a Polyclonal antibody targeting CA8 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12391-1-AP targets CA8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, Jurkat cells, K-562 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human gliomas tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3068 Product name: Recombinant human CA8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-143 aa of BC015531 Sequence: MADLSFIEDTVAFPEKEEDEEEEEEGVEWGYEEGVEWGLVFPDANGEYQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLSGGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPMEVRIT Predict reactive species Full Name: carbonic anhydrase VIII Calculated Molecular Weight: 290 aa, 32 kDa Observed Molecular Weight: 32-35 kDa GenBank Accession Number: BC015531 Gene Symbol: CA8 Gene ID (NCBI): 767 RRID: AB_2066277 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35219 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.