Proteintech, 12404-1-AP, TPST1 Polyclonal antibody

Size: 20ul / 150ul The TPST1 (12404-1-AP) by Proteintech is a Polyclonal antibody targeting TPST1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12404-1-AP targets TPST1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, mouse heart tissue, rat heart tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TPST1 is a human tyrosylprotein sulfotransferase that uses 3′phosphoadenosine-5′phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. High TPST1 expression was linked with clinicopathological characteristics and unfavorable prognosis of HNSC patients (PMID: 16859706). In lung cancer, TPST1 expression was associated with the tumor-node metastasis (TNM) stage and lymph node metastasis, suggesting that it may be a useful prognostic indicator for lung cancer patients (PMID: 18243191, 17028309). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3093 Product name: Recombinant human TPST1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 30-371 aa of BC013188 Sequence: ECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE Predict reactive species Full Name: tyrosylprotein sulfotransferase 1 Calculated Molecular Weight: 370 aa, 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC013188 Gene Symbol: TPST1 Gene ID (NCBI): 8460 RRID: AB_2877855 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60507 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.