Proteintech, 12411-1-AP, PI4KA Polyclonal antibody

Size: 20ul / 150ul The PI4KA (12411-1-AP) by Proteintech is a Polyclonal antibody targeting PI4KA in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12411-1-AP targets PI4KA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3062 Product name: Recombinant human PI4KA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1745-2044 aa of BC018120 Sequence: YLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY Predict reactive species Full Name: phosphatidylinositol 4-kinase, catalytic, alpha Calculated Molecular Weight: 2044 aa, 231 kDa Observed Molecular Weight: 231 kDa GenBank Accession Number: BC018120 Gene Symbol: PI4KA Gene ID (NCBI): 5297 RRID: AB_2268237 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42356 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.