Proteintech, 12481-1-AP, COQ6 Polyclonal antibody

Size: 20ul / 150ul The COQ6 (12481-1-AP) by Proteintech is a Polyclonal antibody targeting COQ6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12481-1-AP targets COQ6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse liver tissue, rat liver tissue Positive IHC detected in: mouse ovary tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3164 Product name: Recombinant human COQ6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 151-468 aa of BC014181 Sequence: ALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK Predict reactive species Full Name: coenzyme Q6 homolog, monooxygenase (S. cerevisiae) Calculated Molecular Weight: 468 aa, 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC014181 Gene Symbol: COQ6 Gene ID (NCBI): 51004 RRID: AB_10644143 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2Z9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.