Proteintech, 12508-1-AP, MAGEA4 Polyclonal antibody

Size: 20ul / 150ul The MAGEA4 (12508-1-AP) by Proteintech is a Polyclonal antibody targeting MAGEA4 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, U2OS cells Positive IHC detected in: human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3193 Product name: Recombinant human MAGEA4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC017723 Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV Predict reactive species Full Name: melanoma antigen family A, 4 Calculated Molecular Weight: 317 aa, 35 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC017723 Gene Symbol: MAGEA4 Gene ID (NCBI): 4103 RRID: AB_2877863 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43358 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.