Proteintech, 12520-1-AP, TRUB1 Polyclonal antibody

Size: 20ul / 150ul The TRUB1 (12520-1-AP) by Proteintech is a Polyclonal antibody targeting TRUB1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 12520-1-AP targets TRUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HepG2 cells Positive IP detected in: mouse liver tissue Positive IHC detected in: mouse kidney tissue, human kidney tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3213 Product name: Recombinant human TRUB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC030601 Sequence: MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC Predict reactive species Full Name: TruB pseudouridine (psi) synthase homolog 1 (E. coli) Calculated Molecular Weight: 349 aa, 37 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC030601 Gene Symbol: TRUB1 Gene ID (NCBI): 142940 RRID: AB_10640437 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WWH5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.