Proteintech, 12586-1-AP, SAMHD1 Polyclonal antibody

Size: 20ul / 150ul The SAMHD1 (12586-1-AP) by Proteintech is a Polyclonal antibody targeting SAMHD1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 12586-1-AP targets SAMHD1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue Positive IP detected in: K-562 cells Positive IHC detected in: human lung cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3287 Product name: Recombinant human SAMHD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC036450 Sequence: MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQVCSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKLLSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEIVSNKRNGIDVDKWDY Predict reactive species Full Name: SAM domain and HD domain 1 Calculated Molecular Weight: 626 aa, 72 kDa Observed Molecular Weight: 64-72 kDa GenBank Accession Number: BC036450 Gene Symbol: SAMHD1 Gene ID (NCBI): 25939 RRID: AB_2183496 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y3Z3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.