Proteintech, 12617-1-AP, GNAI1 Polyclonal antibody

Size: 20ul / 150ul The GNAI1 (12617-1-AP) by Proteintech is a Polyclonal antibody targeting GNAI1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12617-1-AP targets GNAI1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, fetal human brain tissue, rat brain tissue Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human kidney tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3290 Product name: Recombinant human GNAI1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC026326 Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVAAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF Predict reactive species Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 Calculated Molecular Weight: 354 aa, 40 kDa Observed Molecular Weight: 38-40 kDa GenBank Accession Number: BC026326 Gene Symbol: GNAI1 Gene ID (NCBI): 2770 RRID: AB_2111346 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P63096 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.