Proteintech, 12653-1-AP, ARMC8 Polyclonal antibody

Size: 20ul / 150ul The ARMC8 (12653-1-AP) by Proteintech is a Polyclonal antibody targeting ARMC8 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12653-1-AP targets ARMC8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HepG2 cells Positive IHC detected in: human cervical cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Armadillo repeat-containing protein 8 (Armc8) is a key factor to regulate cell membrane adhesion complex through promoting α-catenin degradation. Armc8 expression was elevated during atypia-to-carcinoma progression and may be associated with cancer development of breast carcinoma (PMID: 25119601). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3374 Product name: Recombinant human ARMC8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC013424 Sequence: MEVTASSRHYVDRLFDPDPQKVLQGVIDMKNAVIGNNKQKANLIVLGAVPRLLYLLQQETSSTELKTECAVVLGSLAMGTENNVKSLLDCHIIPALLQGLLSPDLKFIEACLRCLRTIFTSPVTPEELLYTDATVIPHLMALLSRSRYTQEYICQIFSHCCKGPDHQTILFNHGAVQNIAHLLTSLSYKVRMQALKCFSVLAFENPQVSMTLVNVLVDGELLPQIFVKMLQRDKPIEMQLTSAKCLTYMCRAGAIRTDDNCIVLKTLPCLVRMCSKERLLEERVEGAETLAYLIEPDVELQRIASITDHLIAMLADYFKYPSSVSAITDIKRLDHDLKHAHELRQAAFKL Predict reactive species Full Name: armadillo repeat containing 8 Calculated Molecular Weight: 673 aa, 75.5 kDa Observed Molecular Weight: 75.5 kDa GenBank Accession Number: BC013424 Gene Symbol: ARMC8 Gene ID (NCBI): 25852 RRID: AB_2059154 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IUR7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.