Proteintech, 12680-1-AP, Protrudin Polyclonal antibody

Size: 20ul / 150ul The Protrudin (12680-1-AP) by Proteintech is a Polyclonal antibody targeting Protrudin in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3369 Product name: Recombinant human ZFYVE27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 217-411 aa of BC030621 Sequence: VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK Predict reactive species Full Name: zinc finger, FYVE domain containing 27 Calculated Molecular Weight: 411 aa, 46 kDa Observed Molecular Weight: 46-50 kDa GenBank Accession Number: BC030621 Gene Symbol: ZFYVE27 Gene ID (NCBI): 118813 RRID: AB_10640298 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5T4F4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.