Proteintech, 12712-1-AP, GCNF Polyclonal antibody

Size: 20ul / 150ul The GCNF (12712-1-AP) by Proteintech is a Polyclonal antibody targeting GCNF in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12712-1-AP targets GCNF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, K-562 cells, mouse testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3395 Product name: Recombinant human NR6A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC030600 Sequence: MERDEPPPSGGGGGGGSAGFLEPPAALPPPPRNGFCQDELAELDPGTNDRAEQRTCLICGDRATGLHYGIISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNRCQYCRLLKCLQMGMNRKAIREDGMPGGRNKSIGPVQISEEEIERIMSGQEFEEEANHWSNHGDSDHSSPGNRASESNQPSPGSTLSSRSVELNGFMAFREQYMGMSVPPHYQYIPHLFSYSGHSPLLPQQARSLDPQSYSLIHQLLSAEDLEPLGTPMLIEDGYAVTQAELFALLCRLADELLFRQIAWIKKLPFFCELSIKDYTCLLSSTWQELILLSSLTVYSKQIFGELADVTAKYSPSD Predict reactive species Full Name: nuclear receptor subfamily 6, group A, member 1 Calculated Molecular Weight: 475 aa, 54 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: BC030600 Gene Symbol: GCNF Gene ID (NCBI): 2649 RRID: AB_10640439 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15406 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.