Proteintech, 12771-1-AP, MAFF Polyclonal antibody

Size: 20ul / 150ul The MAFF (12771-1-AP) by Proteintech is a Polyclonal antibody targeting MAFF in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12771-1-AP targets MAFF in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human lung tissue Positive IP detected in: SKOV-3 cells Positive IHC detected in: human ovary tumor tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:15000-1:22000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3460 Product name: Recombinant human MAFF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC015037 Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS Predict reactive species Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) Calculated Molecular Weight: 17 aa, 8 kDa Observed Molecular Weight: 18-23 kDa GenBank Accession Number: BC015037 Gene Symbol: MAFF Gene ID (NCBI): 23764 RRID: AB_2137677 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULX9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.