Proteintech, 12797-1-AP, HSPA2 Polyclonal antibody

Size: 20ul / 150ul The HSPA2 (12797-1-AP) by Proteintech is a Polyclonal antibody targeting HSPA2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12797-1-AP targets HSPA2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human normal colon, human ovary tumor tissue, human prostate hyperplasia tissue, human testis tissue, mouse ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3539 Product name: Recombinant human HSPA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 290-639 aa of BC036107 Sequence: LYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEEVD Predict reactive species Full Name: heat shock 70kDa protein 2 Calculated Molecular Weight: 639 aa, 70 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC036107 Gene Symbol: HSPA2 Gene ID (NCBI): 3306 RRID: AB_2119687 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P54652 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.