Proteintech, 12825-1-AP, MLLT3/AF9 Polyclonal antibody

Size: 20ul / 150ul The MLLT3/AF9 (12825-1-AP) by Proteintech is a Polyclonal antibody targeting MLLT3/AF9 in WB, ELISA applications with reactivity to human, mouse, rat samples 12825-1-AP targets MLLT3/AF9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HL-60 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information MLLT3, also named as AF9, YEATS3, is a 568 amino acid protein, which is a component of the superelongation complex6 and co-operates with DOT1L, which di/trimethylates H3K79 to promote transcription. MLLT3 localizes to active transcription start sites (TSSs) through the YEATS domain, which recognizes active histone marks such as H3K9 acetylation and crotonylation. A truncated MLLT3 that lacks the YEATS domain forms a leukaemic fusion protein with the N terminus of MLL1, which misdirects MLLT3-interacting complexes to induce aberrant gene transcription. MLLT3 also regulates erythroid or megakaryocytic progenitors and was identified as a definitive HSC hub gene during mouse development. Researcher found that the expression of a gene called MLLT3 was closely correlated with blood stem cells'potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. It does this by working with other regulatory proteins to keep important parts of the blood stem cell's machinery operational as the cells divide. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3528 Product name: Recombinant human MLLT3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC036089 Sequence: MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKK Predict reactive species Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 Calculated Molecular Weight: 568 aa, 63 kDa Observed Molecular Weight: 63-70 kDa GenBank Accession Number: BC036089 Gene Symbol: AF9/MLLT3 Gene ID (NCBI): 4300 RRID: AB_2145949 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42568 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.